Mouse Anti-MICA/B Recombinant Antibody (PABC-516) (CAT#: PABC-516)
This product is a recombinant mouse antibody that can recognize MICA and MICB.
Specific Inquiry
SDS-PAGE
Figure 1 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in SDS-PAGE
SDS-PAGE analysis of PABC-516 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of PABC-516 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in HPLC
The purity of PABC-516 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in WB
Western blot analysis of PABC-516 was performed by loading recombinant MICA protein.
PABC-516 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in ELISA
ELISA analysis of PABC-516 was performed by coating with recombinant MICA protein. Then blocked with BSA and incubated with Mouse Anti-MICA/B Recombinant Antibody (PABC-516). The Goat Anti-mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- MIC protein(s)
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human MICA/B
- Species Reactivity
- Human
- Applications
- ELISA
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.
Target
- Alternative Names
- MICA; MICB; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B
- Gene ID
- 100507436
- UniProt ID
- Q29983
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-517 | Mouse Anti-MICA/B Recombinant Antibody (PABC-517) | ELISA, Inhib | Mouse IgG2a |
| FN-173CQ | Recombinant Mouse Anti-MICA/B Antibody (6D4) | FC, FuncS, IHC, IP | Mouse IgG2a, κ |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-516, RRID: AB_3111596)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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