Mouse Anti-MICA/B Recombinant Antibody (PABC-516)
CAT#: PABC-516
This product is a recombinant mouse antibody that can recognize MICA and MICB.
Tested Data
SDS-PAGE
Figure 1 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in SDS-PAGE
SDS-PAGE analysis of PABC-516 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of PABC-516 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in HPLC
The purity of PABC-516 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in WB
Western blot analysis of PABC-516 was performed by loading recombinant MICA protein.
PABC-516 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Mouse Anti-MICA/B Recombinant Antibody (PABC-516) in ELISA
ELISA analysis of PABC-516 was performed by coating with recombinant MICA protein. Then blocked with BSA and incubated with Mouse Anti-MICA/B Recombinant Antibody (PABC-516). The Goat Anti-mouse IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- MIC protein(s)
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human MICA/B
- Species Reactivity
- Human
- Applications
- ELISA
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.
Target
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABC-516, RRID: AB_3111596)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "MICA/B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-517 | Mouse Anti-MICA/B Recombinant Antibody (PABC-517) | ELISA, Inhib | Mouse IgG2a |
| FN-173CQ | Recombinant Mouse Anti-MICA/B Antibody (6D4) | FC, FuncS, IHC, IP | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBC-516 | Mouse Anti-MICA/B Recombinant Antibody Fab Fragment (PFBC-516) | ELISA | Mouse Fab |
| PFBC-517 | Mouse Anti-MICA/B Recombinant Antibody Fab Fragment (PFBC-517) | ELISA, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-516 | Mouse Anti-MICA/B Recombinant Antibody scFv Fragment (PSBC-516) | ELISA | Mouse scFv |
| PSBC-517 | Mouse Anti-MICA/B Recombinant Antibody scFv Fragment (PSBC-517) | ELISA, Inhib | Mouse scFv |
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